Phosphoprotein Component of Vaccinia Virions
نویسندگان
چکیده
منابع مشابه
Early promoter-binding factor from vaccinia virions.
A factor, present in transcriptionally active extracts prepared from purified vaccinia virus particles, binds to vaccinia early promoter sequences. The specificity of binding was demonstrated by electrophoretic mobility shift assays using the 5'-terminal segments of two early genes and related and unrelated competitor DNA fragments. DNase I "footprint" analysis indicated that the factor formed ...
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The protein synthesis inhibitor previously demonstrated to be associated with vaccinia cores was quantitatively solubilized from vaccinia virions or cores after an endogenous protein kinase reaction at pH 10. The presence of the inhibitor in the soluble extract correlated with the presence of soluble virion proteins phosphorylated in vitro. Partially purified inhibitor blocked methionyl-tRNAfMe...
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Morphogenesis of vaccinia virus begins with the appearance of crescent-shaped membrane precursors of immature virions in cytoplasmic factories. During the initial characterization of the product of the L2R reading frame, we discovered that it plays an important role in crescent formation. The L2 protein was expressed early in infection and was associated with the detergent-soluble membrane frac...
متن کاملPurification and characterization of a DNA-dependent RNA polymerase from vaccinia virions.
A DNA-dependent RNA polymerase has been extracted from vaccinia virions and purified to near homogeneity as judged by glycerol gradient sedimentation and polyacrylamide gel electrophoresis. The native enzyme has a molecular weight of approximately 500,000 and can be dissociated into putative subunits of 140,000, 137,000, 37,000, 35,000, 31,000, 22,000, and 17,000 daltons. Activity was dependent...
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Modified vaccinia virus Ankara (MVA) is a highly attenuated vaccinia virus strain developed during the smallpox eradication campaign. Nowadays recombinant attenuated poxviruses gain importance as live carrier vaccines against different infectious diseases and in cancer therapy. The aim of this work was to develop recombinant viral vectors, for presentation of a foreign antigen on the surface of...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1973
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.11.6.961-970.1973